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A) Representative images of Aβ immunohistochemistry. The dashed lines indicate boundaries of either the cortex or hippocampus. Scale bar, 500 μm. B) Levels of soluble Aβ42 measured by ELISA in hippocampus homogenate. C, D) Quantification of plaque density in hippocampus (C) and cortex (D), measured as the total Aβ+ plaque area relative to total area. E, F) Frequency distribution analysis based on plaque size in hippocampus (E) and cortex (F). G) Representative images of CA1 microglia expressing IBA1 (red) and <t>CD68</t> (green). Scale bar, 100 μm. H) Left, quantification of the number of microglia (IBA1+ cells) per field of view (0.5 mm 2 ). Right, quantification of mean CD68 intensity per field of view. Statistical analysis performed with one-way ANOVA (B, C, D, H) or two-way ANOVA (E, F); n = 5-7 mice per genotype. See table S3 for full statistical details. *p<0.05, ***p<0.001, ****p<0.0001, ns: not significant. Data are presented as mean ± SEM.
Cd68, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kp1/CD68%2FSR-D1+Antibody+(KP1)+-+BSA+Free/bio_rxiv__64898__2026__03__24__713865-289-32-34
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A) Representative images of Aβ immunohistochemistry. The dashed lines indicate boundaries of either the cortex or hippocampus. Scale bar, 500 μm. B) Levels of soluble Aβ42 measured by ELISA in hippocampus homogenate. C, D) Quantification of plaque density in hippocampus (C) and cortex (D), measured as the total Aβ+ plaque area relative to total area. E, F) Frequency distribution analysis based on plaque size in hippocampus (E) and cortex (F). G) Representative images of CA1 microglia expressing IBA1 (red) and <t>CD68</t> (green). Scale bar, 100 μm. H) Left, quantification of the number of microglia (IBA1+ cells) per field of view (0.5 mm 2 ). Right, quantification of mean CD68 intensity per field of view. Statistical analysis performed with one-way ANOVA (B, C, D, H) or two-way ANOVA (E, F); n = 5-7 mice per genotype. See table S3 for full statistical details. *p<0.05, ***p<0.001, ****p<0.0001, ns: not significant. Data are presented as mean ± SEM.
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fitc plus anti human cd68 kp1 mouse igg2a recombinant antibody  (Proteintech)
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A) Representative images of Aβ immunohistochemistry. The dashed lines indicate boundaries of either the cortex or hippocampus. Scale bar, 500 μm. B) Levels of soluble Aβ42 measured by ELISA in hippocampus homogenate. C, D) Quantification of plaque density in hippocampus (C) and cortex (D), measured as the total Aβ+ plaque area relative to total area. E, F) Frequency distribution analysis based on plaque size in hippocampus (E) and cortex (F). G) Representative images of CA1 microglia expressing IBA1 (red) and <t>CD68</t> (green). Scale bar, 100 μm. H) Left, quantification of the number of microglia (IBA1+ cells) per field of view (0.5 mm 2 ). Right, quantification of mean CD68 intensity per field of view. Statistical analysis performed with one-way ANOVA (B, C, D, H) or two-way ANOVA (E, F); n = 5-7 mice per genotype. See table S3 for full statistical details. *p<0.05, ***p<0.001, ****p<0.0001, ns: not significant. Data are presented as mean ± SEM.
Fitc Plus Anti Human Cd68 Kp1 Mouse Igg2a Recombinant Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc plus anti human cd68 kp1 mouse igg2a recombinant antibody - by Bioz Stars, 2026-10
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anti cd68  (Novus Biologicals)
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A) Representative images of Aβ immunohistochemistry. The dashed lines indicate boundaries of either the cortex or hippocampus. Scale bar, 500 μm. B) Levels of soluble Aβ42 measured by ELISA in hippocampus homogenate. C, D) Quantification of plaque density in hippocampus (C) and cortex (D), measured as the total Aβ+ plaque area relative to total area. E, F) Frequency distribution analysis based on plaque size in hippocampus (E) and cortex (F). G) Representative images of CA1 microglia expressing IBA1 (red) and <t>CD68</t> (green). Scale bar, 100 μm. H) Left, quantification of the number of microglia (IBA1+ cells) per field of view (0.5 mm 2 ). Right, quantification of mean CD68 intensity per field of view. Statistical analysis performed with one-way ANOVA (B, C, D, H) or two-way ANOVA (E, F); n = 5-7 mice per genotype. See table S3 for full statistical details. *p<0.05, ***p<0.001, ****p<0.0001, ns: not significant. Data are presented as mean ± SEM.
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anti cd68 af700  (Novus Biologicals)
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A) Representative images of Aβ immunohistochemistry. The dashed lines indicate boundaries of either the cortex or hippocampus. Scale bar, 500 μm. B) Levels of soluble Aβ42 measured by ELISA in hippocampus homogenate. C, D) Quantification of plaque density in hippocampus (C) and cortex (D), measured as the total Aβ+ plaque area relative to total area. E, F) Frequency distribution analysis based on plaque size in hippocampus (E) and cortex (F). G) Representative images of CA1 microglia expressing IBA1 (red) and <t>CD68</t> (green). Scale bar, 100 μm. H) Left, quantification of the number of microglia (IBA1+ cells) per field of view (0.5 mm 2 ). Right, quantification of mean CD68 intensity per field of view. Statistical analysis performed with one-way ANOVA (B, C, D, H) or two-way ANOVA (E, F); n = 5-7 mice per genotype. See table S3 for full statistical details. *p<0.05, ***p<0.001, ****p<0.0001, ns: not significant. Data are presented as mean ± SEM.
Anti Cd68 Af700, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Representative images of Aβ immunohistochemistry. The dashed lines indicate boundaries of either the cortex or hippocampus. Scale bar, 500 μm. B) Levels of soluble Aβ42 measured by ELISA in hippocampus homogenate. C, D) Quantification of plaque density in hippocampus (C) and cortex (D), measured as the total Aβ+ plaque area relative to total area. E, F) Frequency distribution analysis based on plaque size in hippocampus (E) and cortex (F). G) Representative images of CA1 microglia expressing IBA1 (red) and CD68 (green). Scale bar, 100 μm. H) Left, quantification of the number of microglia (IBA1+ cells) per field of view (0.5 mm 2 ). Right, quantification of mean CD68 intensity per field of view. Statistical analysis performed with one-way ANOVA (B, C, D, H) or two-way ANOVA (E, F); n = 5-7 mice per genotype. See table S3 for full statistical details. *p<0.05, ***p<0.001, ****p<0.0001, ns: not significant. Data are presented as mean ± SEM.

Journal: bioRxiv

Article Title: Deletion of astrocyte intermediate filaments GFAP and Vimentin enhances protein synthesis and prevents early synaptic and cognitive dysfunction in a mouse model of Alzheimer’s disease

doi: 10.64898/2026.03.24.713865

Figure Lengend Snippet: A) Representative images of Aβ immunohistochemistry. The dashed lines indicate boundaries of either the cortex or hippocampus. Scale bar, 500 μm. B) Levels of soluble Aβ42 measured by ELISA in hippocampus homogenate. C, D) Quantification of plaque density in hippocampus (C) and cortex (D), measured as the total Aβ+ plaque area relative to total area. E, F) Frequency distribution analysis based on plaque size in hippocampus (E) and cortex (F). G) Representative images of CA1 microglia expressing IBA1 (red) and CD68 (green). Scale bar, 100 μm. H) Left, quantification of the number of microglia (IBA1+ cells) per field of view (0.5 mm 2 ). Right, quantification of mean CD68 intensity per field of view. Statistical analysis performed with one-way ANOVA (B, C, D, H) or two-way ANOVA (E, F); n = 5-7 mice per genotype. See table S3 for full statistical details. *p<0.05, ***p<0.001, ****p<0.0001, ns: not significant. Data are presented as mean ± SEM.

Article Snippet: Antibodies were used against GFAP (1:1000, Dako, #Z0334 or 1:2000, SynapticSystems, #173004), VIM (1:4000, Sigma-Aldrich, #5733 or 1:1000, Abcam, #ab92547), S100β (1:250, Sigma-Aldrich, #S2532 or 1:600, Dako, #Z0311), IBA1 (1:500, SySy, #234308), CD68 (1:100, Novus Biologicals, #NB100-683), Aβ-6E10 (1:1000, ITK Diagnostic, #SIG-39320) FUS (1:1000, Life Technologies, #A300-293A), hnRNPL (1:1000, Santa Cruz, #sc-32317) and HA (1:750, Roche, #11867423001).

Techniques: Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Expressing